Transmembrane signaling protocols

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Since the publication of the first edition, new advances have increased the ability to understand transmembrane signaling processes in greater detail. The human genome has been completely sequenced as well as new methods have been developed for mircroarrays and proteomics analyzing global changes in gene expression and protein profiles. Updated to include these new methods, the 2nd edition also includes comprehensive detailed methods, studies in genetically tractable systems, fluorescence microscopy in live single cells, ex vivo analysis of primary cells from transgenic mice, and genomic and proteomic approaches to the analysis of transmembrane signaling events.